本教材是针对新形势下的教学改革，为适应中国与国际接轨，高等院校普及双语教学的需要而编写的。本教材根据医学免疫学与微生物学的教学要求，遵循实用性、科学性的原则，从实验目的、材料、方法、结果分析及思考题等方面入手，较为系统和全面地介绍医学免疫学及医学微生物学的基本实验技术。全教材分为两篇：第一篇为医学免疫学实验；第二篇为医学微生物学实验。本教材培养学生独立操作、观察和思考，分析问题和解决实际问题的能力。

江振友,男,1963年9月生，暨南大学医学院微生物学与免疫学系系主任、教授、博士生导师。中国微生物学会医学微生物学与免疫学专业委员会委员；广东省免疫学会第四届理事会理事；广东省防痨协会第八届理事会常务理事；广东省微生物学会第九届理事会理事。主要从事分子病毒学、感染免疫、病原生物感染诊断研究。近年发表论文60余篇，其中SCI收录20多篇。主编或参编著作5部。获得各类科研研究奖励4项，教学研究奖励6项。主持承担国家、省部级等各类科研课题6项,研究经费300多万元。授权发明专利3项。

前言

Preface
Part1MedicalImmunologyExperiment
Instructions
i．PurposesandRequirementsoftheExperiment
ii．RulesandRegulationsoftheLaboratory
I．AgglutinationReaction
i．DirectAgglutinationReaction
ii．IndirectAgglutinationReaction
II．PrecipitationReaction·
i．SingleAgarImmunodiffusionTest
ii．DoubleAgarhnmunodiffusionTest
iii．CounterImmunoelectrophoresisTest
iv．ImmunoelectrophoresisTest
v．RocketElectrophoresisTest
III．PhagocytosisTest
i．ThePhagoeytosisofMacrophage--BigPhagocytosisPhenomenon
ii．NeutrophilPhagocytosis--SmallPhagocytosisPhenomenon(InVitro)
IV．ThePreparationofMouseSplenocyte
V．HemolyticPlaqueFormationTest
VI．E-RosetteFormingCellTest
VII.TLymphocyteTransformationTest
VIII.ImmunolabellingTechnique
i．DetectionofBLymphocyteAntigenRecognitionReceptors(Smlg)(DirectImmunofluorescenceMethod
ii．Enzyme-LinkedImmunosorbentAssay(ELISA)
IX．AnaphylacticShockinGuineaPig
Part2MedicalMicrobiologyExperiment
X．MorphologyandStructureoftheBacteria
XI．ObservationofUnstainedBacteriaSpecimen
XII．ObservationofStainedBacteriaSpecimen
i．GramsStaining
ii．Acid-FastStaining
XIII．ArtificiaICultureofBacteria
i．InoculationMethodsforArtificialCultureofBacteria
ii．BiochemicalReactionofBacteria
XIV.TheDistributionOfBacteria
i．BacterialExaminationintheNaturalEnvironment
ii．NormalHumanBacterialExamination
XV．TheInfluenceofExternalFactorsonBacteria
i．TheInfluenceofPhysicalFactorsonBacteria
ii．TheInfluenceofChemicalFactorsonBacteria
iii．Test0nBacterialSensitivitytoAntibiotics(AgarDiffusionMethod)
XVI．TheSpecificBacteriolyticTestofBacteriophage
XVII．PathogenicityTestofPathogenicBacteria
i．DiffusionEffectofHyaluronidase
ii．PlasmaCoagulaseTest
iii．DetectionofEndotoxinandLimulusTest
XVIII．LysozymeTest
XIX．PathogenicCocci
i．TheMorphologyandStainingCharacteristicsofStaphylococcus，Streptococcus，StreDtococcMsPneumoniae，NeisseriaMeningitidisandGonococcus
ii．TheCultureCharacteristicsofStaphylococcus，StreptococcusandStreptococcusPneumoniae
iii．Anti-StreptolysinO(ASO)Detection
XX．Enterobacteriaceae
i．TheMorphologyandStainingCharacteristicsofEnterobacteriaceae
ii．CultureCharacteristicsofEnterobacteriaceae
iii．BiochemicalReactionsofEnterobacteriaceae
XXI．TheSeparationandIdentificationofPathogenicEnterobacteriaceaeinFecesSpecimens
XXII．TubeAgglutination
XXIII．AnaerobicClostridium
i．AnaerobicCuhure
ii．MorphologyandStainingCharacteristicsofClostridiumTetani，ClostridiumPerfringensandClostridiumBotulinum
XXIV．Mycobacterium
i．TheCuitureCharacteristicsofMycobacteriumTuberculosis
ii．DirectSmearExaminationofSputumSamplesofPulmonaryTuberculosisPatients
XXV．TheMorphologyofSpirochetes(Demo)
XXVI．BasicMorphologyandColonyCharacteristicsofFungi
XXVII．MorphologicalCharacteristicsofViruses
i．ObservationofViralCPE
ii．ObservationofViralInclusionBody
XXVIII．CulturaIMethodsofViruses
i．ChickEmbryoCultureMethod
ii．CellCultureMethod
XXIX．HemagglutinationandHemagglutinationInhibitionTestofInfluenzaVirus
i．HemagglutinationTest
ii．HemagglutinationInhibitionTest