本书是在1994年郭勇主编的中国轻工业出版社出版的《酶工程》和2004年科学出版社出版的《酶工程》(第二版)的基础上，根据国内外酶工程的最新进展，结合笔者的教学实践和科研成果，重新编写修改补充而成的‘十一五’国家级规划教材。本书主要介绍酶的生产、改性和应用的基本理论、基本技术及其最新进展和发展趋势。内容包括绪论，微生物发酵产酶，动植物细胞培养产酶，酶的提取与分离纯化，酶分子修饰，酶、细胞和原生质体固定化，酶的非水相催化，酶定向进化,酶反应器，酶的应用等九十章。每章都附有复习思考题，书末附有中英文专业名。

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Contents
Preface
Chapter1 Introduction 1
1.1 Conception of Enzyme and Its Development 2
1.2 The Characteristics of Enzyme Action 3
1.3 Factors that Affecting Enzyme Action 7
1.4 Classification and Nomenc1ature of Enzymes 11
1.5 Determination of Enzyme Activity 19
1.6 Methods for Enzymes Production 24
1.7 Development Survey of Enzyme Engineering 28
Review and Thinking 32
Chapter 2 Enzyme Production by Microbial Fermentation33
2.1 Regulation of Enzyme Biosynthesis in Microbial Cells 34
2.2 Characteristic of Microbes for Enzyme Production 49
2.3 Fermentation Process Conditions and Its Connol 53
2.4 Enzyme Fermentation Kinetics 65
2.5 Enzyme Production by Immobilized Cell Fermentation 71
2.6 Enzyme Production by Innnobilized Protoplast Fermentation 78
Review and Thinking 80
Chapter 3 Enzyme Production by Animal and Plant CelI Cultures 81
3.1 The Regulation of Enzyme Biosynthesis in Animal and Plant Cells 82
3.2 Enzyme Production by Plant Cell Culture 86
3.3 Enzyme Production by Animal Cell Culture 97
Review and Thinking ..105
Chapter 4 Extraction, Separation and purification，tioo of Enzymes 106
4.1 Cell Disruption 106
4.2 Enzyme Extraction 110
4.3 Precipitation Separation 113
4.4 Centrifugal Separation 118
4.5 Filtration and Menlbrane Separation 124
4.6 Chrornatographic Separation 130
4.7 Electrophoresis Separation 150
4.8 Exnaction Separation 160
4.9 Crystallization 167
4.10 Concentration and Drying 170
Review and Thinking 172
Chapter 5 Enzyme Molecular Modification 174
5.1 Metal Ion Substitution Modification 175
5.2 Macromolecular Binding Modification 177
5.3 Side Chain Group Modification 181
5.4 Peptide Chain Limited Hydrolysis Modification 187
5.5 Nucleotide Chain Cleavage Modification 188
5.6 Amino Acid Substitution Modification 189
5.7 Nucleotide Substitution Modification 192
5.8 Physical Modification 193
5.9 Application of Enzyme Molecular Modification 194
Review and Thlnking 200
Chapter 6 ImmobilizatiOll of Enzymes, cells and Protoplasts 201
6.1 Enzyme Immobilization 203
6.2 Cell Immo biliza tion 216
6.3 Protoplast Immobilization 228
Review and Thlnking 231
Chapter 7 Enzyme Catalysis in Non-aqueous phases 232
7.1 The Main Scopes of Enzyme Catalysis in Non-aqueous Phase 233
7.2 Effects of Water and Organic Solvents on Enzyme Catalysis in Organic Media 234
7.3 The Catalytic Characters of Enzymes in Organic Media 240
7.4 Conditions of Enzyme Catalytic Reaction in Organic Media and Its Control 244
7.5 Application of Enzyme Catalysis in Non-aqueous Phase 251
Review and Thlnking 260
Chapter 8 Enzyme Directed Evolotion 261
8.1 The characteristics of Enzyme Directed Evolution 263
8.2 Random Mutation of Enzyme Gene 264
8.3 Directed Selection of Enzyme Gene Mutants 270
8.4 The Applications of Enzyme Directed Evolution 281
Review and Thlnking 283
Chapter 9 Enzyme Reactors 285
9.1 Types of Enzyme Reactors 286
9.2 Choice of Enzyme Reactors 292
9.3 Design of Enzyme Reactors 296
9.4 Enzyme Reactor Operation 301
Review and Thlnking 306
Chapter 10 Application of Enzymes 307
10.1 Enzymes Application in Pharmaceutical Industry 307
10.2 Application of Enzymes in Food 328
10.3 Enzyme Application in Light and Chemical Industries 342
10.4 Enzyme Application in Environmental Protection 349
10.5 Enzyme Application in Biotechnology 352
Review and Thinking 358
Main references 359