本书主要介绍了干细胞与再生医学的相关研究进展，包括干细胞的基础研究与衍生研究，及其在不同生理和病理水平中的治疗作用。全书共分九章，内容包括：干细胞生物学概述、干细胞的分离与鉴定、干细胞与人类疾病、干细胞/干细胞微环境与组织再生、肿瘤干细胞、类器官、不同类型的类器官、细胞外囊泡、基因编辑与基因治疗。

Chapter 1 Introduction to Stem Cell Biology 第一章 干细胞生物学概述

1 Stem Cell Research Timeline--Highlights

1.1 Stem Cell Research History

1.2 The Turning Point in Stem Cell Therapy

2 Introduction of Regenerative Medicine and Stem Cells

3 Types and Origin of Stem Cell

3.1 Stem Cell Classification Based on Differentiation Potential

3.2 Stem Cell Classification Based on Origin

4 Stem Cells and Their Niches

5 Application of Stem Cells

5.1 Stem Cells Used in Regenerative Medicine

5.2 Stem Cells for Drug Screening and Development in Laboratory

5.3 A Muhidisciplinary Field

6 Challenges and Requirements

Supplement

References

Chapter 2 Isolation and Identification of Stem Cells 第二章 干细胞的分离与鉴定

1 Embryonic Stem Cells

1.1 Genetic Modification Approaches and Potential Applications

1.2 Methods for Genetic Manipulation

2 Mesenchymal Stem Cells

2.1 Mesenchymal Stem Cells Derived from Human Adipose Tissue

2.2 Mesenchymal Stem Cells from Human Bone Marrow

3 Induced Pluripotent Stem Cells

3.1 Integrating Vectors

3.2 Non-DNA Reprogramming

4 Aduit Neural Stem Ceils

4.1 Neural Stem Cells：Origins and Functions

4.2 AduIt Brains Can Generate New Neurons

4.3 AduIt Neural Stem Cells：Implications for Therapy

5 Experimental Method

5.1 Materials

5.2 Methods

Supplement

References

Chapter 3 Stem Cells and Human Diseases 第三章 干细胞与人类疾病

1 Stem Cell Therapy for Neurological Disorders

1.1 Neural Stem Cells

1.2 Hematopoietic Stem Cells

1.3 Mesenchymal Stem Cells

2 Stem Cell Therapy in Heart Diseases

2.1 Stem Cell—Based Therapies

2.2 Brief Summary

3 Stem Cell Therapy in Diabetes Mellitus

3.1 B-Cell Replacement

3.2 Embryonic Stem Cells

3.3 Induced Pluripotent Stem Cells

3.4 Mesenchymal Stem Cells

3.5 Brief Summary

4 Stem Cell Therapy in Osteoarthritis

4.1 Stem Cell Therapy

4.2 Route of Administration

5 Stem Cell Therapy in Wound Healing

5.1 Stages of Wound

5.2 Chronic Wounds

5.3 Types of Stem Cells

5.4 Brief Summary

Supplement

References

Chapter 4 Stem Cell／Stem Cell Niche and Tissue Regeneration 第四章 干细胞／干细胞微环境与组织再生

1 Introduction to Stem Cell Niche

1.1 Definition

1.2 Components of the Niche

1.3 Niche Factors

1.4 Recap

2 Introduction to Tissue Engineering for Regeneration Medicine

2.1 Manipulating of the Niche for Regenerative

2.2 The Design of Stem Cell Niche

2.3 Synthetic Niches in Vivo

2.4 Ligand Immobilization

2.5 Tissue Engineering

2.6 Scaffold

2.7 Question

Supplement

Rerefences

Chapter 5 Cancer Stem Cells 第五章 肿瘤干细胞

1 Introduction to Cancer Stem Cells

1.1 Definition

1.2 Characteristics

1.3 Mechanism of Cancer Stem Cell Resistance to Therapy

1.4 Origin of Cancer Stem Cells

1.5 Characterize Cancer Stem Cells

1.6 Cancer Stem Cells Markers

1.7 Isolation of Cancer Stem Cells

1.8 The Correlation Between Cancer Stem Cells.Epithelial-Mesenchymal Transition and Vasculogenic Mimicy Formation

2 The Cancer Stem Cell Niche

2.1 Niche Components

2.2 Niche Fartots

2.3 Cancer Stem CeUs Derived from Mouse Induced Pluripotent Stem Cells When Exposed to a Malignant Niche

2.4 Importance of Cancer Stem Cell Niche

3 Challenges and Approaches in Cancer Stem Ceils Research

3.1 Challenges

3.2 Approaches in Cancer Stem Cells Research

Supplement

References

Chapter 6 Organoids 第六章 类器官

1 Introduction to Organoids

1.1 Definition

1.2 Development of Organoids

1.3 Origin of Organoids

1.4 The Culture of Organoids

1.5 The Composition of Organoids

1.6 Identification of Organoids

1.7 The Comparison of Organoids wit